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algorithms incucyte s3 software sartorius prism 10 graphpad software flowjo bd compass  (Sartorius AG)


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    Sartorius AG algorithms incucyte s3 software sartorius prism 10 graphpad software flowjo bd compass
    Algorithms Incucyte S3 Software Sartorius Prism 10 Graphpad Software Flowjo Bd Compass, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 15969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flowjo+analysis+software/Live+Cell+Analysis+Instruments/pmc12490239__mmc8-200-17-21
    Average 99 stars, based on 15969 article reviews
    algorithms incucyte s3 software sartorius prism 10 graphpad software flowjo bd compass - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: Design of a fragment crystallizable-engineered tetravalent bispecific antibody targeting programmed cell death-1 and vascular endothelial growth factor with cooperative biological effects.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LDH assay kit Roche 11644793001 ELISA Dakewe 1110202,1110002 DynabeadsTM M280 anti-mouse IgG Fc beads Thermofisher 11202D Experimental models: Organisms/strains SCID/Beige mice Beiing Vital River Laboratory AnimalTechnology Co., Ltd. N/A C57BL/6-hPD-1/hPD-L1/hCD73 transgenic mice GemPharmatech Co., Ltd N/A Cynomolgus monkeys (M. fascicularis) Guangxi Guidong Quadrumana Development&Laboratory Co. Ltd. (Guigang, Guangxi, CHN) Guangxi Grandforest Scientific Primate Company (Guigang, Guangxi, CHN), Ltd. Beijing Primas Biotech Co. Ltd. (Beijing, CHN) N/A Experimental models: Cell lines 293T cells Wuhan University N/A 293T-PD1 cells Akeso Biopharma N/A Jurkat cells Chinese Academy of Sciences SCSP-513 Jurkat-PD-1 cells Akeso Biopharma N/A HUVECs Allcells H-001F-C PD-L1 aAPC/CHO-K1 cells Promega J112A PD-1 effector cells Promega J108A 293T-KDR-NFAT-LUC cells Akeso Biopharma N/A Raji cells Chinese Academy of Sciences, Shanghai Branch TCHu 44 Raji-PD-L1 cells Akeso Biopharma N/A Jurkat-NFAT-CD64-CD32H cells Akeso Biopharma N/A HCC827 JENNIO Biological N/A .. MC38-hPD-L1/hCD73 cells GemPharmatech N/A CHO-K1-PD-1 cells Akeso Biopharma N/A Software and algorithms Fortebio Octet molecular interaction system Pall/Fortebio Model: Octet QKe Fortebio Data Acquisition 7.0 N/A Fortebio Data Analysis 7.0 N/A Ficoll-PaqueTM Plus GE 17-1440-02 Prism 8.0 GraphPad https://www.graphpad.com/feature FlowJo_V10 BD Biosciences https://www.flowjo.com/ .. HUVECs (human umbilical vein endothelial cell) were cultured in HUVEC cell culture medium (Allcells, H-004).

    Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells
    Article Snippet: .. Luca Grumolato Jun 2, 2025 ChemiDoc Imaging System (Bio-Rad), QuantStudio Flex PCR System (Thermo Scientific), Beckman Coulter CytoFLEX, Infinite F200 PRO (TECAN), Illumina MiniSeq, Incucyte® S3 Live-Cell Analysis System (Sartorius), Axioscope 7 microscope, Exigo H400 system Prism 4 software from GraphPad (GraphPad Software Inc), Microsoft Excel LTSC MSO (16.0.14332.21031) 64 bits, Image Lab Software (version 6.0.1; Bio-Rad), QuantStudio Design and Analysis Software (v1.4.3; Thermo Scientific), CytExpert software (v2.5.0.77; Beckman Coulter), Imaris software (v10.0.2; Bitplane), Incucyte software (v2021C; Sartorius), FlowJo software (V10.10.0, Becton Dickinson). re p o rtfo lio | rep o rtin g su m m ary A pril2023 Data Policy information about availability of data All manuscripts must include a data availability statement. ..

    Article Title: Combining BET inhibition with SMAC mimetics restricts tumor growth and triggers immune surveillance in preclinical cancer models
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CellTiter-Glo®2.0 Promega G9242 GAPDH assay kit MSD MSD-K151PWD-2 cIAP1 assay kit MSD F217V Qiagen RNeasy Lipid Tissue Kit Qiagen 74804 TaqMan Fast Advanced Master Mix Applied Biosystems 4444556 Quantseq 3′mRNA-Seq Library Prep Kit-FWD Lexogen 015 QuBit 1x dsDNA HS Assay Kit Invitrogen Q33231 High Sensitivity D1000 Reagents Agilent 5067–5585 High Sensitivity DNA ScreenTape Agilent 5067–5584 Kapa Library Quantification Kit ROX low Roche KK4973 Tumor Dissociation Kit, mouse Miltenyi 130-096-730 CD45 (TIL) MicroBeads, mouse Miltenyi 130-110-618 BD Ms Single Cell Sample Multiplexing Kit BD 633793 BD Rhapsody Cartridge Reagent Kit BD 633731 BD Rhapsody Cartridge Kit BD 633733 BD Rhapsody cDNA Kit BD 633773 BD Rhapsody Immune Response Panel Mm BD 633753 BD Rhapsody Targeted mRNA and AbSeq Amplification Kit BD 633774 Deposited data Single cell sequencing data GEO accession number GSE269602 Experimental models: Cell lines Full list of human cell lines and culture conditions is given in Table S1. .. Experimental models: Organisms/strains BomTac:NMRI-Foxn1nu mice Taconic Biosciences C57BL/6NTac mice Taconic Biosciences NOD SCID Axis Bioservices Software and algorithms Incucyte® S3 Software Sartorius Prism 10 GraphPad Software FlowJo BD Compass for Simple Western ProteinSimple version 6.1 SevenBridges platform version 1.9.1 Seurat package version 4.2.0 Scrublet version 0.2.3 SingleR package version 2.0.0 Cell Reports Medicine 6, 102313, September 16, 2025 e3 Article ll OPEN ACCESS Observational Trial (NCT04120935, IFOMCPO003/2018/PO002) or the ‘‘Analisi farmacogenomica di campioni tumorali’’ protocol (version 2, dated January 14th 2010). ..

    Article Title: Non-canonical activating roles of RCoR2 sustain transcription in adrenergic neuroblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER TrimGalore (v. 0.6.7) Babraham Bioinformatics 73 https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ HISAT2 (v. 2.2.1) Kim et al. 74 N/A StringTie (v2.1.7) Pertea et al. 75 N/A R package DESeq2 Love et al. 76 N/A Nextflow nf-core/atacseq pipeline (v. 2.1.2) nf-core 77 https://github.com/nf-core/atacseq HiC-Pro (v. 2.11.1) Servant et al. 78 N/A Juicebox Durand et al. 79 N/A GraphPad Prism-GraphPad Software (v. 9.5.1) GraphPad Software Inc RRID: SCR_002798 ImageJ ImageJ N/A GSEA (Broad Institute, v.4.3.2) Subramanian et al., Mootha et al. 80,81 N/A Incucyte® Software Sartorius N/A FlowJo TM BD Life Sciences N/A R2 genomics R2 genomics https://r2.amc.nl/ R (v.4.3.0) R Development Core Team https://www.R-project.org/ 20 Cell Reports ▪▪, 115951, ▪▪, 2025 OPEN ACCESS Whole cell lysates, nuclear extract and western blots To confirm RCOR1 and RCOR2 knockdown, whole-cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 2 mM Phenylmethylsulfonyl fluoride (PMSF), 1X Roche Complete Protease Inhibitor cocktail), while, because of an intense cytosolic unspecific band, RCoR3 levels were detected on nuclear extract. .. In particular, cells were washed in ice-cold PBS, resuspended in Ipotonic Buffer (10 mM HEPES, 50 mM NaCl, 0.5 mM DTT, 1 mM NaPiro, 1 mM NaOrtho, 1 mM NaFluor, 1 mM PMSF, 1X Roche Complete Protease Inhibitor cocktail) and pelleted by centrifugation at 300g for 5 min.

    Article Title: Combining BET inhibition with SMAC mimetics restricts tumor growth and triggers immune surveillance in preclinical cancer models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CellTiter-Glo®2.0 Promega G9242 GAPDH assay kit MSD MSD-K151PWD-2 cIAP1 assay kit MSD F217V Qiagen RNeasy Lipid Tissue Kit Qiagen 74804 TaqMan Fast Advanced Master Mix Applied Biosystems 4444556 Quantseq 3 ′ mRNA-Seq Library Prep Kit-FWD Lexogen 015 QuBit 1x dsDNA HS Assay Kit Invitrogen Q33231 High Sensitivity D1000 Reagents Agilent 5067–5585 High Sensitivity DNA ScreenTape Agilent 5067–5584 Kapa Library Quantification Kit ROX low Roche KK4973 Tumor Dissociation Kit, mouse Miltenyi 130-096-730 CD45 (TIL) MicroBeads, mouse Miltenyi 130-110-618 BD Ms Single Cell Sample Multiplexing Kit BD 633793 BD Rhapsody Cartridge Reagent Kit BD 633731 BD Rhapsody Cartridge Kit BD 633733 BD Rhapsody cDNA Kit BD 633773 BD Rhapsody Immune Response Panel Mm BD 633753 BD Rhapsody Targeted mRNA and AbSeq Amplification Kit BD 633774 Deposited data Single cell sequencing data GEO accession number GSE269602 Experimental models: Cell lines Full list of human cell lines and culture conditions is given in Table S1. .. Experimental models: Organisms/strains BomTac:NMRI-Foxn1 nu mice Taconic Biosciences C57BL/6NTac mice Taconic Biosciences NOD SCID Axis Bioservices Software and algorithms Incucyte® S3 Software Sartorius Prism 10 GraphPad Software FlowJo BD Compass for Simple Western ProteinSimple version 6.1 SevenBridges platform version 1.9.1 Seurat package version 4.2.0 Scrublet version 0.2.3 SingleR package version 2.0.0 Cell Reports Medicine 6, 102313, September 16, 2025 e3 OPEN ACCESS Observational Trial (NCT04120935, IFOMCPO003/2018/PO002) or the ‘‘Analisi farmacogenomica di campioni tumorali’’ protocol (version 2, dated January 14th 2010). ..

    Imaging:

    Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells
    Article Snippet: .. Luca Grumolato Jun 2, 2025 ChemiDoc Imaging System (Bio-Rad), QuantStudio Flex PCR System (Thermo Scientific), Beckman Coulter CytoFLEX, Infinite F200 PRO (TECAN), Illumina MiniSeq, Incucyte® S3 Live-Cell Analysis System (Sartorius), Axioscope 7 microscope, Exigo H400 system Prism 4 software from GraphPad (GraphPad Software Inc), Microsoft Excel LTSC MSO (16.0.14332.21031) 64 bits, Image Lab Software (version 6.0.1; Bio-Rad), QuantStudio Design and Analysis Software (v1.4.3; Thermo Scientific), CytExpert software (v2.5.0.77; Beckman Coulter), Imaris software (v10.0.2; Bitplane), Incucyte software (v2021C; Sartorius), FlowJo software (V10.10.0, Becton Dickinson). re p o rtfo lio | rep o rtin g su m m ary A pril2023 Data Policy information about availability of data All manuscripts must include a data availability statement. ..

    Polymerase Chain Reaction:

    Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells
    Article Snippet: .. Luca Grumolato Jun 2, 2025 ChemiDoc Imaging System (Bio-Rad), QuantStudio Flex PCR System (Thermo Scientific), Beckman Coulter CytoFLEX, Infinite F200 PRO (TECAN), Illumina MiniSeq, Incucyte® S3 Live-Cell Analysis System (Sartorius), Axioscope 7 microscope, Exigo H400 system Prism 4 software from GraphPad (GraphPad Software Inc), Microsoft Excel LTSC MSO (16.0.14332.21031) 64 bits, Image Lab Software (version 6.0.1; Bio-Rad), QuantStudio Design and Analysis Software (v1.4.3; Thermo Scientific), CytExpert software (v2.5.0.77; Beckman Coulter), Imaris software (v10.0.2; Bitplane), Incucyte software (v2021C; Sartorius), FlowJo software (V10.10.0, Becton Dickinson). re p o rtfo lio | rep o rtin g su m m ary A pril2023 Data Policy information about availability of data All manuscripts must include a data availability statement. ..

    Microscopy:

    Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells
    Article Snippet: .. Luca Grumolato Jun 2, 2025 ChemiDoc Imaging System (Bio-Rad), QuantStudio Flex PCR System (Thermo Scientific), Beckman Coulter CytoFLEX, Infinite F200 PRO (TECAN), Illumina MiniSeq, Incucyte® S3 Live-Cell Analysis System (Sartorius), Axioscope 7 microscope, Exigo H400 system Prism 4 software from GraphPad (GraphPad Software Inc), Microsoft Excel LTSC MSO (16.0.14332.21031) 64 bits, Image Lab Software (version 6.0.1; Bio-Rad), QuantStudio Design and Analysis Software (v1.4.3; Thermo Scientific), CytExpert software (v2.5.0.77; Beckman Coulter), Imaris software (v10.0.2; Bitplane), Incucyte software (v2021C; Sartorius), FlowJo software (V10.10.0, Becton Dickinson). re p o rtfo lio | rep o rtin g su m m ary A pril2023 Data Policy information about availability of data All manuscripts must include a data availability statement. ..

    Simple Western:

    Article Title: Combining BET inhibition with SMAC mimetics restricts tumor growth and triggers immune surveillance in preclinical cancer models
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CellTiter-Glo®2.0 Promega G9242 GAPDH assay kit MSD MSD-K151PWD-2 cIAP1 assay kit MSD F217V Qiagen RNeasy Lipid Tissue Kit Qiagen 74804 TaqMan Fast Advanced Master Mix Applied Biosystems 4444556 Quantseq 3′mRNA-Seq Library Prep Kit-FWD Lexogen 015 QuBit 1x dsDNA HS Assay Kit Invitrogen Q33231 High Sensitivity D1000 Reagents Agilent 5067–5585 High Sensitivity DNA ScreenTape Agilent 5067–5584 Kapa Library Quantification Kit ROX low Roche KK4973 Tumor Dissociation Kit, mouse Miltenyi 130-096-730 CD45 (TIL) MicroBeads, mouse Miltenyi 130-110-618 BD Ms Single Cell Sample Multiplexing Kit BD 633793 BD Rhapsody Cartridge Reagent Kit BD 633731 BD Rhapsody Cartridge Kit BD 633733 BD Rhapsody cDNA Kit BD 633773 BD Rhapsody Immune Response Panel Mm BD 633753 BD Rhapsody Targeted mRNA and AbSeq Amplification Kit BD 633774 Deposited data Single cell sequencing data GEO accession number GSE269602 Experimental models: Cell lines Full list of human cell lines and culture conditions is given in Table S1. .. Experimental models: Organisms/strains BomTac:NMRI-Foxn1nu mice Taconic Biosciences C57BL/6NTac mice Taconic Biosciences NOD SCID Axis Bioservices Software and algorithms Incucyte® S3 Software Sartorius Prism 10 GraphPad Software FlowJo BD Compass for Simple Western ProteinSimple version 6.1 SevenBridges platform version 1.9.1 Seurat package version 4.2.0 Scrublet version 0.2.3 SingleR package version 2.0.0 Cell Reports Medicine 6, 102313, September 16, 2025 e3 Article ll OPEN ACCESS Observational Trial (NCT04120935, IFOMCPO003/2018/PO002) or the ‘‘Analisi farmacogenomica di campioni tumorali’’ protocol (version 2, dated January 14th 2010). ..

    Article Title: Combining BET inhibition with SMAC mimetics restricts tumor growth and triggers immune surveillance in preclinical cancer models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CellTiter-Glo®2.0 Promega G9242 GAPDH assay kit MSD MSD-K151PWD-2 cIAP1 assay kit MSD F217V Qiagen RNeasy Lipid Tissue Kit Qiagen 74804 TaqMan Fast Advanced Master Mix Applied Biosystems 4444556 Quantseq 3 ′ mRNA-Seq Library Prep Kit-FWD Lexogen 015 QuBit 1x dsDNA HS Assay Kit Invitrogen Q33231 High Sensitivity D1000 Reagents Agilent 5067–5585 High Sensitivity DNA ScreenTape Agilent 5067–5584 Kapa Library Quantification Kit ROX low Roche KK4973 Tumor Dissociation Kit, mouse Miltenyi 130-096-730 CD45 (TIL) MicroBeads, mouse Miltenyi 130-110-618 BD Ms Single Cell Sample Multiplexing Kit BD 633793 BD Rhapsody Cartridge Reagent Kit BD 633731 BD Rhapsody Cartridge Kit BD 633733 BD Rhapsody cDNA Kit BD 633773 BD Rhapsody Immune Response Panel Mm BD 633753 BD Rhapsody Targeted mRNA and AbSeq Amplification Kit BD 633774 Deposited data Single cell sequencing data GEO accession number GSE269602 Experimental models: Cell lines Full list of human cell lines and culture conditions is given in Table S1. .. Experimental models: Organisms/strains BomTac:NMRI-Foxn1 nu mice Taconic Biosciences C57BL/6NTac mice Taconic Biosciences NOD SCID Axis Bioservices Software and algorithms Incucyte® S3 Software Sartorius Prism 10 GraphPad Software FlowJo BD Compass for Simple Western ProteinSimple version 6.1 SevenBridges platform version 1.9.1 Seurat package version 4.2.0 Scrublet version 0.2.3 SingleR package version 2.0.0 Cell Reports Medicine 6, 102313, September 16, 2025 e3 OPEN ACCESS Observational Trial (NCT04120935, IFOMCPO003/2018/PO002) or the ‘‘Analisi farmacogenomica di campioni tumorali’’ protocol (version 2, dated January 14th 2010). ..

    Western Blot:

    Article Title: Non-canonical activating roles of RCoR2 sustain transcription in adrenergic neuroblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER TrimGalore (v. 0.6.7) Babraham Bioinformatics 73 https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ HISAT2 (v. 2.2.1) Kim et al. 74 N/A StringTie (v2.1.7) Pertea et al. 75 N/A R package DESeq2 Love et al. 76 N/A Nextflow nf-core/atacseq pipeline (v. 2.1.2) nf-core 77 https://github.com/nf-core/atacseq HiC-Pro (v. 2.11.1) Servant et al. 78 N/A Juicebox Durand et al. 79 N/A GraphPad Prism-GraphPad Software (v. 9.5.1) GraphPad Software Inc RRID: SCR_002798 ImageJ ImageJ N/A GSEA (Broad Institute, v.4.3.2) Subramanian et al., Mootha et al. 80,81 N/A Incucyte® Software Sartorius N/A FlowJo TM BD Life Sciences N/A R2 genomics R2 genomics https://r2.amc.nl/ R (v.4.3.0) R Development Core Team https://www.R-project.org/ 20 Cell Reports ▪▪, 115951, ▪▪, 2025 OPEN ACCESS Whole cell lysates, nuclear extract and western blots To confirm RCOR1 and RCOR2 knockdown, whole-cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 2 mM Phenylmethylsulfonyl fluoride (PMSF), 1X Roche Complete Protease Inhibitor cocktail), while, because of an intense cytosolic unspecific band, RCoR3 levels were detected on nuclear extract. .. In particular, cells were washed in ice-cold PBS, resuspended in Ipotonic Buffer (10 mM HEPES, 50 mM NaCl, 0.5 mM DTT, 1 mM NaPiro, 1 mM NaOrtho, 1 mM NaFluor, 1 mM PMSF, 1X Roche Complete Protease Inhibitor cocktail) and pelleted by centrifugation at 300g for 5 min.

    Knockdown:

    Article Title: Non-canonical activating roles of RCoR2 sustain transcription in adrenergic neuroblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER TrimGalore (v. 0.6.7) Babraham Bioinformatics 73 https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ HISAT2 (v. 2.2.1) Kim et al. 74 N/A StringTie (v2.1.7) Pertea et al. 75 N/A R package DESeq2 Love et al. 76 N/A Nextflow nf-core/atacseq pipeline (v. 2.1.2) nf-core 77 https://github.com/nf-core/atacseq HiC-Pro (v. 2.11.1) Servant et al. 78 N/A Juicebox Durand et al. 79 N/A GraphPad Prism-GraphPad Software (v. 9.5.1) GraphPad Software Inc RRID: SCR_002798 ImageJ ImageJ N/A GSEA (Broad Institute, v.4.3.2) Subramanian et al., Mootha et al. 80,81 N/A Incucyte® Software Sartorius N/A FlowJo TM BD Life Sciences N/A R2 genomics R2 genomics https://r2.amc.nl/ R (v.4.3.0) R Development Core Team https://www.R-project.org/ 20 Cell Reports ▪▪, 115951, ▪▪, 2025 OPEN ACCESS Whole cell lysates, nuclear extract and western blots To confirm RCOR1 and RCOR2 knockdown, whole-cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 2 mM Phenylmethylsulfonyl fluoride (PMSF), 1X Roche Complete Protease Inhibitor cocktail), while, because of an intense cytosolic unspecific band, RCoR3 levels were detected on nuclear extract. .. In particular, cells were washed in ice-cold PBS, resuspended in Ipotonic Buffer (10 mM HEPES, 50 mM NaCl, 0.5 mM DTT, 1 mM NaPiro, 1 mM NaOrtho, 1 mM NaFluor, 1 mM PMSF, 1X Roche Complete Protease Inhibitor cocktail) and pelleted by centrifugation at 300g for 5 min.

    Protease Inhibitor:

    Article Title: Non-canonical activating roles of RCoR2 sustain transcription in adrenergic neuroblastoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER TrimGalore (v. 0.6.7) Babraham Bioinformatics 73 https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ HISAT2 (v. 2.2.1) Kim et al. 74 N/A StringTie (v2.1.7) Pertea et al. 75 N/A R package DESeq2 Love et al. 76 N/A Nextflow nf-core/atacseq pipeline (v. 2.1.2) nf-core 77 https://github.com/nf-core/atacseq HiC-Pro (v. 2.11.1) Servant et al. 78 N/A Juicebox Durand et al. 79 N/A GraphPad Prism-GraphPad Software (v. 9.5.1) GraphPad Software Inc RRID: SCR_002798 ImageJ ImageJ N/A GSEA (Broad Institute, v.4.3.2) Subramanian et al., Mootha et al. 80,81 N/A Incucyte® Software Sartorius N/A FlowJo TM BD Life Sciences N/A R2 genomics R2 genomics https://r2.amc.nl/ R (v.4.3.0) R Development Core Team https://www.R-project.org/ 20 Cell Reports ▪▪, 115951, ▪▪, 2025 OPEN ACCESS Whole cell lysates, nuclear extract and western blots To confirm RCOR1 and RCOR2 knockdown, whole-cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 2 mM Phenylmethylsulfonyl fluoride (PMSF), 1X Roche Complete Protease Inhibitor cocktail), while, because of an intense cytosolic unspecific band, RCoR3 levels were detected on nuclear extract. .. In particular, cells were washed in ice-cold PBS, resuspended in Ipotonic Buffer (10 mM HEPES, 50 mM NaCl, 0.5 mM DTT, 1 mM NaPiro, 1 mM NaOrtho, 1 mM NaFluor, 1 mM PMSF, 1X Roche Complete Protease Inhibitor cocktail) and pelleted by centrifugation at 300g for 5 min.



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